Scientific School 2018
The School
Nutritional metabolomics has recently appeared as a holistic and high throughput approach to identify and characterize biochemical pathways that underlie multifaceted associations between dietary exposures and chronic diseases with altered metabolic phenotypes. Compared to a foodomics, related just to the food metabolite characterization before consumption, nutritional metabolomics has strongly emerged to study the effects of dietary compounds on human metabolism after consumption, and/or to identify dietary intake biomarkers.
Epidemiologic studies suggest that dietary patterns can potentially significantly lower the risk of certain diseases such as cancer, cardiovascular disease, Alzheimer’s disease and more recently Parkinson’s disease.
The Mediterranean diet rich in natural antioxidants, vitamins, and phytochemicals are perceived as healthful by Western countries, but there is a limited scientific proof that dietary compounds can affect or perturbate the human metabolism.
The ability to identify new correlations between dietary habits and health, or between consumption of specific foods and disease-related outcomes has presented important challenges due metabolite identification and to individual variability in complex metabolic pathways and digestion.
Themes
Participants will learn about:
- How to design a metabolomic experiment;
- How to measure nutritional and metabolism biomarkers;
- How to find correlations between dietary habits and health.
Who should attend
The School is targeted at graduate students and early-stage researchers in metabolomics and bioinformatics. Participants should have some metabolomics experience. Some statistical data analysis experience will be helpful, but not strictly required.
Lecturers
Cristina Andres-Lacueva, Nutrition, Food Science & Gastronomy Department, University of Barcelona, Spain. CIBER of Frailty and Healthy Aging (CIBERFES)
Andrea Armirotti , ITT, Genova, Italy
Luigi Atzori Department of Biomedical Sciences, Oncology and Molecular Pathology Unit, University of Cagliari, Italy
Lorraine Brennan – Institute of Food and Health and Conway Institute, UCD School of Agriculture and Food Science, Dublin, Ireland
Pierluigi Caboni, Department of Life and Environmental Sciences, High Resolution Mass Spectrometry Laboratory, Food Toxicology Unit, University of Cagliari, Italy
Marina Cocchi, University of Modena and Reggio Emilia, Italy
Lars Ove Dragsted, University of Copenhagen, Denmark
Pietro Franceschi, Fondazione Edmund Mach, Trento, Italy
Hector Gallart Ayala, UNIL Metabolomics University of Lausanne, Switzerland
Jules Griffin , University of Cambridge, UK
Julijana Ivanisevic, UNIL Metabolomics University of Lausanne, Switzerland
Cristina Piras , University of Cagliari, Italy
Serge Rezzi , Nestlé Institute of Health Sciences, Lausenne, Switzerland
Reza Salek, International Agency for Research on Cancer, WHO, France
Paola Scano, Department of Chemical and Geological Sciences, University of Cagliari – Istituto Studio Macromolecole, CNR, Milano, Italy
Matteo Stocchero , University of Padova, Italy
Marynka Ulaszewska , Fondazione Edmund Mach, Trento, Italy
David Wishart , University of Alberta, Canada
Format
The course is scheduled to begin on the afternoon of Monday Oct. 1st 2018; Tue-Thu will be full course days, while Friday Oct. 5th will be a half day, with the course finishing at lunch time.
- First two days hands on activities (tutorials), introductory lectures ;
- Third and fourth day lectures and presentations.
Under the tutors guidance, the practical sessions will give students the opportunity to learn and design metabolomic experiments. Participants will be able to develop nutritional metabolomics studies.
Students will have ample time to meet and speak with lecturers, especially at the coffee breaks, lunches and social events.
The organizing committee:
- Pierluigi Caboni, University of Cagliari
- Luigi Atzori, University of Cagliari
- Enrico Pieroni, CRS4
- Jules Griffin, University of Cambridge
Local organization
- Emanuela Falqui (CRS4) – Image Coordination and Publicity
- Michela Grimal (TOXMED) – Project Management and Organizing secretariat
None of this would be possible without the generous financial support of Sardegna Ricerche and the Autonomous Region of Sardinia.
Web
Partners
- Università degli Studi Cagliari
- Sardegna Ricerche
- Regione Autonoma della Sardegna
Program
October 1st
15.00 – 16.00 Registration
16.00 – 16.15 Openings: Prof. Pierluigi Caboni – Prof. Luigi Atzori, University of Cagliari, Italy
16.15 – 17.15 Plenary lecture – Metabolomics and the Dream of Precision Nutrition – Prof. David Wishart, University of Alberta, Canada
17.15 – 18.30 Welcome cocktail at Sardegna ricerche
October 2nd
9.00 – 9.45 Lecture – Metabolomics and Dietary Biomarkers – Prof. Lorraine Brennan, Institute of Food and Health and Conway Institute, UCD School of Agriculture and Food Science, Dublin, Ireland.
9.45-10.30 Lecture – Analytical trends in Human Nutrition – Dr. Serge Rezzi, Nestlé Institute of Health Sciences, Lausanne, Switzerland
10.30 – 11.00 Lecture – MS based metabolomics: an introduction Mass spectrometry – Prof. Pietro Franceschi, Fondazione Edmund Mach (TN) Italy
11.00 – 11.30 Coffee break
11.30 – 12.00 Lecture – Data Analysis Strategies – Prof. Marina Cocchi, Università degli Studi di Modena e Reggio Emilia, Italy
12.00 – 12.30 Lecture – Data analysis: PLS in metabolomics – Prof. Matteo Stocchero, University of Padua, Italy
12.30 – 12.45 Introduction to hands on activity – Dr. Marynka Ulaszewska, Fondazione Edmund Mach (TN), Italy
12.45 – 13.00 Introduction to hands on activity NMR spectroscopy experiments in metabolomics – Dr. Cristina Piras, University of Cagliari, Italy
13 – 14.15 Lunch
14.30 – 16.00 Tutorial activity – Annotations from A to Z: theory and practice for LC-MS based metabolomics (Bring with you spectra you wish to annotate!!) – Dr. Marynka Ulaszewska, Fondazione Edmund Mach (TN), Italy
16.00 -17.30 Tutorial activity – part 1 – NMR spectroscopy experiments in metabolomics – Dr. Cristina Piras, Department of Biomedical Sciences, Oncology and Molecular Pathology Unit, University of Cagliari, Italy
October 3rd
9.00 – 9.45 Lecture – Nutritional metabolomics and the study of dietary patterns in Clinical & Epidemiological studies: beyond the Mediterranean Diet – Prof. Cristina Andres-Lacueva, Nutrition, Food Science & Gastronomy Department, University of Barcelona, Spain. CIBER of Frailty and Healthy Aging (CIBERFES)
9.45 – 10.30 Lecture – Precision Nutrition- the role for metabolomics – Prof. Lorraine Brennan – Institute of Food and Health and Conway Institute, UCD School of Agriculture and Food Science, Dublin, Ireland
10.30 – 11.00 Lecture – Overview of International data standards and repository – Prof. Reza Salek, International Agency for Research on Cancer, WHO, France
11.00 – 11.30 Coffee break
11.30 – 12.15 Lecture – Biomarker classification and validation from a metabolomics perspective – Prof. Lars Ove Dragsted, University of Copenhagen, Denmark
12.15 – 13.00 Lecture – Ion Mobility for Untargeted Lipidomics – Dr. Andrea Armirotti, IIT Genova, Italy
13 – 14.15 Lunch
14.30 – 16.00 Tutorial activity – part 2 –NMR spectroscopy in metabolomics application – Prof. Paola Scano, Department of Chemical and Geological Sciences, University of Cagliari, Italy
16.00 -17.30 Tutorial activity – Mass spectrometry – Mass Spectrometry strategies for advanced lipid profiling – Dr. Hector Gallart Ayala, UNIL Metabolomics University of Lausanne, Switzerland
October 4th
9.00 – 9.45 Lecture – Odd sample types and multiple profiling – Prof. Lars Ove Dragsted, University of Copenhagen, Denmark
9.45 – 10.30 Lecture – In silico Metabolomics and Novel Compound Identification in Nutrition Studies – Prof. David Wishart, University of Alberta, Canada.
10.30 – 11.00 Lecture – Pre-analytical factors and their influence on analytical quality in nutrimetabolomics – Dr. Marynka Ulaszewska, Fondazione Edmund Mach (TN), Italy
11.00 – 11.30 Coffee break
11.30 – 12.15 Lecture – Workflow for metabolomics data and pathway analysis using XCMS Online – Prof. Julijana Ivanisevic, UNIL Metabolomics University of Lausanne, Switzerland
12.15 – 12.45 Lecture – Do these Branch Chain Amino Acids make my bum look big? And other stories from metabolomics and human intervention studies – Prof. Jules Griffin, University of Cambridge, U.K.
13 – 14.15 Lunch
14.30 – 16.00 Tutorial activity – Projection to latent structures with orthogonal constraints: structured noise and data fusion – Prof. Matteo Stocchero, University of Padua, Italy
20.00 Social dinner at Bacchixeddu Restaurant (Pula)
October 5th
9.00 – 9.30 Lecture – Beyond biomarkers and towards mechanisms – Prof. Julijana Ivanisevic – UNIL Metabolomics University of Lausanne, Switzwerland
9.30 – 10.00 Lecture – Exposome and Cancer metabolomics – Prof. Reza Salek, International Agency for Research on Cancer, WHO, France
10.00 – 10.30 Lecture – Metabolomics in complex experimental designs. Are we ready for that? – Prof. Pietro Franceschi, Fondazione Edmund Mach (TN), Italy
11.00 – 11.30 Coffee break
11.00 – 11.30 Lecture – What to measure in metabolomics? – Prof. Luigi Atzori Department of Biomedical Sciences, Oncology and Molecular Pathology Unit, University of Cagliari, Italy
11.30 – 12.00 Lecture – Iodine deficiency during pregnancy – Prof. Pierluigi Caboni, Department of Life and Environmental Sciences, High Resolution Mass Spectrometry Laboratory, Food Toxicology Unit, University of Cagliari, Italy
12.00 – 12.30 – Conclusions
13 – 14 Lunch