![\"\"](\"http:\/\/sites.unica.it\/pe2026\/files\/2025\/09\/cropped-00_Profile_Tondo-Social__Spilla_PE26_1200px.png\")
<\/figure>FPC10 – Oriana Vitale<\/strong><\/p>\n\n\n\n University of Naples Federico II, Italy<\/p>\n\n\n\n oriana.vitale@unina.it<\/strong><\/p>\n<\/div><\/div>\n\n\n\n <\/p>\n\n\n\n <\/p>\n","protected":false},"excerpt":{"rendered":" FPC10 – Oriana Vitale University of Naples Federico II, Italy oriana.vitale@unina.it Comparative Study between Cholesterol and its Natural Derivative on RNA-lipid Interactions and Membrane Dynamics in Self-Assembling Lipid Nanoparticles Oriana Vitale1, Antonio De Nicola2, Valeria Nele1, […]<\/p>\n","protected":false},"author":10371,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-2004","post","type-post","status-publish","format-standard","hentry","category-senza-categoria"],"_links":{"self":[{"href":"https:\/\/sites.unica.it\/pe2026\/wp-json\/wp\/v2\/posts\/2004","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.unica.it\/pe2026\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.unica.it\/pe2026\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.unica.it\/pe2026\/wp-json\/wp\/v2\/users\/10371"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.unica.it\/pe2026\/wp-json\/wp\/v2\/comments?post=2004"}],"version-history":[{"count":5,"href":"https:\/\/sites.unica.it\/pe2026\/wp-json\/wp\/v2\/posts\/2004\/revisions"}],"predecessor-version":[{"id":2190,"href":"https:\/\/sites.unica.it\/pe2026\/wp-json\/wp\/v2\/posts\/2004\/revisions\/2190"}],"wp:attachment":[{"href":"https:\/\/sites.unica.it\/pe2026\/wp-json\/wp\/v2\/media?parent=2004"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.unica.it\/pe2026\/wp-json\/wp\/v2\/categories?post=2004"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.unica.it\/pe2026\/wp-json\/wp\/v2\/tags?post=2004"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}Comparative Study between Cholesterol and its Natural Derivative on RNA-lipid Interactions and Membrane Dynamics in Self-Assembling Lipid Nanoparticles<\/strong><\/td><\/tr> Oriana Vitale1<\/sup>, Antonio De Nicola2<\/sup>, Valeria Nele1<\/sup>, Giuseppe De Rosa1<\/sup>, Bruno Catalanotti1<\/sup> and Federica Moraca.1<\/sup><\/em>
1<\/sup><\/em> University of Naples \u201cFederico II\u201d, Department of Pharmacy, via Domenico Montesano, 49, 80131 Naples, Italy.
2<\/sup><\/em> Department of High-Performance Computing, CINECA, Casalecchio di Reno, 40033 Bologna, Italy.<\/td><\/tr>Abstract<\/strong>
Self-assembling nanoparticles (SANPs) are lipid-based nanocarriers composed of a lipid bilayer formed by cationic lipids, sterols, and PEGylated lipids, enclosing a calcium phosphate core capable of encapsulating RNA, demonstrating high efficacy as nucleic acid delivery platforms, particularly in the treatment of glioblastoma [1]. SANPs are typically formed under basic conditions (pH ~9) through a self-assembly process triggered by mixing lipid components with calcium phosphate and ncRNA, leading to the spontaneous formation of organized nanoscale structures. A distinctive feature of these nanoparticles is their organization, often described as micellar or core-shell, which contributes to their stability and encapsulation efficiency [1]. In this context, the role of sterols is crucial. Cholesterol, commonly used in lipid formulations, is known to increase bilayer order and rigidity, thereby enhancing the structural stability of nanoparticles. Cholesterol derivatives, characterized by a bulkier side chain, can alter lipid packing, membrane fluidity, and curvature, thus influencing lateral domain formation and potentially improving RNA encapsulation efficiency [2][3]. To investigate these effects, atomistic molecular dynamics simulations (AA-MD) have been performed on systems containing cholesterol and its natural derivative, both in the presence and absence of RNA. Parameters such as lipid lateral diffusion, order parameters, density profiles, RNA solvent-accessible surface area (SASA), and RNA-lipid distances were analyzed to elucidate the role of sterols in complex stabilization and in the mechanisms governing RNA-membrane interactions [4].<\/td><\/tr>References <\/strong>
[1] Campani V. et al. Int. J. Pharm.<\/em>, 2020<\/strong>, 588, <\/em>119693.
[2] Liu C et al. ACS Appl. Mater. Interfaces,<\/em> 2025<\/strong>, 17<\/em>, 4416-4425.
[3] Back P.I. et al. ACS Nano<\/em>, 2024<\/strong>, 18<\/em>, 28480-28501.
[4] Zimmer D.N. et al.<\/em> J. Phys. Chem. B.,<\/em> 2024<\/strong>, 128<\/em>, 10165-10117.<\/td><\/tr><\/tbody><\/table><\/div><\/figure>\n\n\n\n